LOCUS       P35S-pat1               2830 bp ds-DNA     linear   PAT 27-MAR-2017
DEFINITION  CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF.
ACCESSION   DL476427.1
VERSION     DL476427.1  GI:218736439
KEYWORDS    JP 2008514233-A/27.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Narva,K.E., Olson,P.D., Zhang,J., Sanders,C.D., Wang,J., Zhong,G.,
            Moellenbeck,D., Meyer,S.E., Luckring,A.K., Bing,J.W., Jr,R.F.C.,
            Hakimi,S.M., Gupta,M., Hondred,D., Krone,T.L. and Locke,M.E.H.
  TITLE     CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF
  JOURNAL   Patent: JP 2008514233-A 27 08-MAY-2008; Pioneer Hi-Bred
            International Inc,Dow AgroSciences LLC, E I DuPont de Nemours and
            Company
COMMENT     OS Artificial Sequence~PN JP 2008514233-A/27~PD 08-MAY-2008~PF
            28-SEP-2005 JP 2007534750~PR 29-SEP-2004 US 60/614225~PI kenneth
            edwin narva,paul d olson,jian zhang,craig d sanders, PI jimei
            wang,~PI gan-yuan zhong,daniel moellenbeck,sandra e meyer,abigail k
            PI luckring,~PI james wayne bing,robert f cressman jr,salim m
            hakimi,manju PI gupta,~PI david hondred,todd l krone,mary e
            hartnett locke CC PCR Amplicon 22I-3.~FH Key Location/Qualifiers.
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="synthetic construct"
                     /mol_type="unassigned DNA"
                     /db_xref="taxon:32630"
     promoter        526..1904
                     /note="p35S; CaMV 35S promoter"
     promoter        1368..1896
     gene            <1872..1896
                     /gene="35S RNA"
     promoter        <1872..1896
                     /gene="35S RNA"
     misc_feature    1897..1915
                     /note="polylinker"
     CDS             1916..>1973
                     /gene="pat"
                     /codon_start=1
                     /transl_table=1
                     /protein_id="CBG92010.1"
                     /db_xref="GI:261382508"
                     /db_xref="UniProtKB/TrEMBL:C9YSI1"
                     /translation="MSPERRPVEIRPATAADMA"
     gene            1916..2467
                     /gene="pat"
     gene            1916..>1973
                     /gene="pat"
ORIGIN      
        1 tacaacctca agtggttcct cttcccgatc gacgacgacc agtacatcat cacctcctac
       61 gccgccaaca actgcaaggt gtggaacgtg aacaacgaca agattaatgt gtcaacctac
      121 tcctccacca actccatcca gaagtggcag atcaaggcca acggctcctc ctacgtgatc
      181 cagtccgaca acggcaaggt gctcaccgcc ggcaccggcc aggccctcgg cctcatccgc
      241 ctcaccgacg agtcctccaa caacccgaac cagcaatgga acctgacgtc cgtgcagacc
      301 atccagctcc cgcagaagcc gatcatcgac accaagctca aggactaccc gaagtactcc
      361 ccgaccggca acatcgacaa cggcacctcc ccgcagctca tgggctggac cctcgtgccg
      421 tgcatcatgg tgaacgaccc gaacatcgac aagaacaccc agatcaagac caccccgtac
      481 tacatcctca agaagtacca gtactggcag agggccgtgg gctccaacgt cgcgctccgc
      541 ccgcacgaga agaagtccta cacctacgag tggggcaccg agatcgacca gaagaccacc
      601 atcatcaaca ccctcggctt ccagatcaac atcgacagcg gcatgaagtt cgacatcccg
      661 gaggtgggcg gcggtaccga cgagatcaag acccagctca acgaggagct caagatcgag
      721 tattcacatg agacgaagat catggagaag taccaggagc agtccgagat cgacaacccg
      781 accgaccagt ccatgaactc catcggcttc ctcaccatca cctccctgga gctctaccgc
      841 tacaacggct ccgagatccg catcatgcag atccagacct ccgacaacga cacctacaac
      901 gtgacctcct acccgaacca ccagcaggcc ctgctgctgc tgaccaacca ctcctacgag
      961 gaggtggagg agatcaccaa catcccgaag tccaccctca agaagctcaa gaagtactac
     1021 ttctgagtca tgagtcatga gtcagttaac ctagacttgt ccatcttctg gattggccaa
     1081 cttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt
     1141 gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc tgaataaaag agaaagagat
     1201 catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag
     1261 atgcatttca ttaaccaaat ccatatacat ataaatatta atcatatata attaatatca
     1321 attgggttag caaaacaaat ctagtctagg tgtgttttgc gaattcccat ggagtcaaag
     1381 attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt
     1441 ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacgctt
     1501 gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt
     1561 caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt
     1621 attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     1681 aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     1741 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     1801 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     1861 tctatataag gaagttcatt tcatttggag aggacagggt acccggggat ccaccatgtc
     1921 tccggagagg agaccagttg agattaggcc agctacagca gctgatatgg ccgcggtttg
     1981 tgatatcgtt aaccattaca ttgagacgtc tacagtgaac tttaggacag agccacaaac
     2041 accacaagag tggattgatg atctagagag gttgcaagat agataccctt ggttggttgc
     2101 tgaggttgag ggtgttgtgg ctggtattgc ttacgctggg ccctggaagg ctaggaacgc
     2161 ttacgattgg acagttgaga gtactgttta cgtgtcacat aggcatcaaa ggttgggcct
     2221 aggatccaca ttgtacacac atttgcttaa gtctatggag gcgcaaggtt ttaagtctgt
     2281 ggttgctgtt ataggccttc caaacgatcc atctgttagg ttgcatgagg ctttgggata
     2341 cacagcccgg ggtacattgc gcgcagctgg atacaagcat ggtggatggc atgatgttgg
     2401 tttttggcaa agggattttg agttgccagc tcctccaagg ccagttaggc cagttaccca
     2461 gatctgagtc gacctgcagg catgcccgct gaaatcacca gtctctctct acaaatctat
     2521 ctctctctat aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     2581 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2641 tctatcaata aaatttctaa ttcctaaaac caaaatccag ggcgagctcg gtacccgggg
     2701 atcctctaga gtcgacctgc aggcatgccc gcggatatcg atgggccccg gccgaagctt
     2761 cggtccgggc catcgtggcc tcttgctctt caggatgaag agctatgttt aaacgtgcaa
     2821 gcgctcaatt
//
