LOCUS       BACVIP3AA               4483 bp ds-DNA     linear   BCT 04-JUN-1996
DEFINITION  Bacillus thuringienis strain AB88 insecticidal protein (vip3A(a))
            gene, complete cds.
ACCESSION   L48811
VERSION     L48811.1  gi:1263317
KEYWORDS    insecticidal protein; vip3A(a) gene.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Estruch, J.J., Warren, G.W., Mullins, M.A., Nye, G.J., Craig, J.A.,
            Koziel, M.G.
  TITLE     Vip3A, a novel Bacillus thuringiensis vegetative insecticidal
            protein with a wide spectrum of activities against lepidopteran
            insects
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 93 (11), 5389-5394 (1996)
  PUBMED    8643585
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="AB88"
                     /db_xref="taxon:1428"
     regulatory      725..732
                     /regulatory_class="ribosome_binding_site"
     gene            739..3108
                     /gene="vip3A(a)"
     CDS             739..3108
                     /gene="vip3A(a)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAC37036.1"
                     /db_xref="GI:1263318"
                     /translation="MNKNNTKLSTRALPSFIDYFNGIYGFATGIKDIMNMIFKTDTGG
                     DLTLDEILKNQQLLNDISGKLDGVNGSLNDLIAQGNLNTELSKEILKIANEQNQVLND
                     VNNKLDAINTMLRVYLPKITSMLSDVMKQNYALSLQIEYLSKQLQEISDKLDIINVNV
                     LINSTLTEITPAYQRIKYVNEKFEELTFATETSSKVKKDGSPADILDELTELTELAKS
                     VTKNDVDGFEFYLNTFHDVMVGNNLFGRSALKTASELITKENVKTSGSEVGNVYNFLI
                     VLTALQAQAFLTLTTCRKLLGLADIDYTSIMNEHLNKEKEEFRVNILPTLSNTFSNPN
                     YAKVKGSDEDAKMIVEAKPGHALIGFEISNDSITVLKVYEAKLKQNYQVDKDSLSEVI
                     YGDMDKLLCPDQSEQIYYTNNIVFPNEYVITKIDFTKKMKTLRYEVTANFYDSSTGEI
                     DLNKKKVESSEAEYRTLSANDDGVYMPLGVISETFLTPINGFGLQADENSRLITLTCK
                     SYLRELLLATDLSNKETKLIVPPSGFISNIVENGSIEEDNLEPWKANNKNAYVDHTGG
                     VNGTKALYVHKDGGISQFIGDKLKPKTEYVIQYTVKGKPSIHLKDENTGYIHYEDTNN
                     NLEDYQTINKRFTTGTDLKGVYLILKSQNGDEAWGDNFIILEISPSEKLLSPELINTN
                     NWTSTGSTNISGNTLTLYQGGRGILKQNLQLDSFSTYRVYFSVSGDANVRIRNSREVL
                     FEKRYMSGAKDVSEMFTTKFEKDNFYIELSQGNNLYGGPIVHFYDVSIK"
ORIGIN      
        1 tctagaccta ggtaaatgag actgtccttc ttatcttaca cgctataatg cggatattaa
       61 ctcaataagc cataaggctt ctaatcgcaa ggactgctgg ggtagttcaa tagctctggt
      121 ataaatagca gtaaaataac ctttggtaca aattcgatag ttactagacg taacgattag
      181 ccgagaaatc cccactttag agagcacgtt aggaggttaa gtgaagggta tttcacaaat
      241 gatcgattat atttatagga aaaggataga tcacttcatc tatagatgaa attatctatc
      301 cttttatttg ttagatagga gatataattt actcatagcc ccagaggaag tgttaacagg
      361 tgatagcatc caattttata gtaagggagg ttttactaag ttctaataat tttctgggta
      421 attagtcctt acaacgactt acatttagga aattgcctgt taataaaatt tatatagtaa
      481 aggagtgctt taatgtaaaa aaatagggat aaatgaattt ttgtgaatag gaagaccatt
      541 tgataggtaa atattataat tccgaaaagt agaataagca aaattgagta acacattaat
      601 gatataaact aattttatac aattgaaatt gataaaaagt tatgagtgtt taataatcag
      661 taattaccaa taaagaatta agaatacaag tttacaagaa ataagtgtta caaaaaatag
      721 ctgaaaagga agatgaacat gaacaagaat aatactaaat taagcacaag agccttacca
      781 agttttattg attattttaa tggcatttat ggatttgcca ctggtatcaa agacattatg
      841 aacatgattt ttaaaacgga tacaggtggt gatctaaccc tagacgaaat tttaaagaat
      901 cagcagttac taaatgatat ttctggtaaa ttggatgggg tgaatggaag cttaaatgat
      961 cttatcgcac agggaaactt aaatacagaa ttatctaagg aaatattaaa aattgcaaat
     1021 gaacaaaatc aagttttaaa tgatgttaat aacaaactcg atgcgataaa tacgatgctt
     1081 cgggtatatc tacctaaaat tacctctatg ttgagtgatg taatgaaaca aaattatgcg
     1141 ctaagtctgc aaatagaata cttaagtaaa caattgcaag agatttctga taagttggat
     1201 attattaatg taaatgtact tattaactct acacttactg aaattacacc tgcgtatcaa
     1261 aggattaaat atgtgaacga aaaatttgag gaattaactt ttgctacaga aactagttca
     1321 aaagtaaaaa aggatggctc tcctgcagat attcttgatg agttaactga gttaactgaa
     1381 ctagcgaaaa gtgtaacaaa aaatgatgtg gatggttttg aattttacct taatacattc
     1441 cacgatgtaa tggtaggaaa taatttattc gggcgttcag ctttaaaaac tgcatcggaa
     1501 ttaattacta aagaaaatgt gaaaacaagt ggcagtgagg tcggaaatgt ttataacttc
     1561 ttaattgtat taacagctct gcaagcccaa gcttttctta ctttaacaac atgccgaaaa
     1621 ttattaggct tagcagatat tgattatact tctattatga atgaacattt aaataaggaa
     1681 aaagaggaat ttagagtaaa catcctccct acactttcta atactttttc taatcctaat
     1741 tatgcaaaag ttaaaggaag tgatgaagat gcaaagatga ttgtggaagc taaaccagga
     1801 catgcattga ttgggtttga aattagtaat gattcaatta cagtattaaa agtatatgag
     1861 gctaagctaa aacaaaatta tcaagtcgat aaggattcct tatcggaagt tatttatggt
     1921 gatatggata aattattgtg cccagatcaa tctgaacaaa tctattatac aaataacata
     1981 gtatttccaa atgaatatgt aattactaaa attgatttca ctaaaaaaat gaaaacttta
     2041 agatatgagg taacagcgaa tttttatgat tcttctacag gagaaattga cttaaataag
     2101 aaaaaagtag aatcaagtga agcggagtat agaacgttaa gtgctaatga tgatggggtg
     2161 tatatgccgt taggtgtcat cagtgaaaca tttttgactc cgattaatgg gtttggcctc
     2221 caagctgatg aaaattcaag attaattact ttaacatgta aatcatattt aagagaacta
     2281 ctgctagcaa cagacttaag caataaagaa actaaattga tcgtcccgcc aagtggtttt
     2341 attagcaata ttgtagagaa cgggtccata gaagaggaca atttagagcc gtggaaagca
     2401 aataataaga atgcgtatgt agatcataca ggcggagtga atggaactaa agctttatat
     2461 gttcataagg acggaggaat ttcacaattt attggagata agttaaaacc gaaaactgag
     2521 tatgtaatcc aatatactgt taaaggaaaa ccttctattc atttaaaaga tgaaaatact
     2581 ggatatattc attatgaaga tacaaataat aatttagaag attatcaaac tattaataaa
     2641 cgttttacta caggaactga tttaaaggga gtgtatttaa ttttaaaaag tcaaaatgga
     2701 gatgaagctt ggggagataa ctttattatt ttggaaatta gtccttctga aaagttatta
     2761 agtccagaat taattaatac aaataattgg acgagtacgg gatcaactaa tattagcggt
     2821 aatacactca ctctttatca gggaggacga gggattctaa aacaaaacct tcaattagat
     2881 agtttttcaa cttatagagt gtatttttct gtgtccggag atgctaatgt aaggattaga
     2941 aattctaggg aagtgttatt tgaaaaaaga tatatgagcg gtgctaaaga tgtttctgaa
     3001 atgttcacta caaaatttga gaaagataac ttttatatag agctttctca agggaataat
     3061 ttatatggtg gtcctattgt acatttttac gatgtctcta ttaagtaaga tcgggatcta
     3121 atattaacag tttttaaaag ctaattcttg tataatgtcc ttgattatgg aaaaacacaa
     3181 ttttgtttgc taagatgtat atatagctca attattaaaa ggctctaaga aaaaacgtat
     3241 ttaaatttaa agttaatgaa ttgtaaggaa aggcacttca ttctcacctc gttatgtcat
     3301 agaagaatta gaatgtttcc cagaaataga taaattatag ttagcgattt gacaaacgaa
     3361 agagaaattc attgtagaca gtttagaatt acgcattata gatgaatagt tttatgcata
     3421 gttgctttaa atgacacagt ataaaagata aacatcactc ttataagagt gatgtttatt
     3481 tttttatact gtgttctaac ataagtgtat agaaaaacat aataaaagta gggggggaag
     3541 tgtagaggta aatgaatatt cttcagtaga agtataatat gatttttagt ccttactaat
     3601 atcagacgaa attaactatc tcataaggct tatattgatg gaggattaaa gtgtttttat
     3661 aacaattggt gaataggtag ctccttcatt tatcatttta gccaagaaga ttccttcctc
     3721 aaggagcgtg aagggtcaca gcacaatgag taggttggag atgaattggc ttgttcaaca
     3781 tacataaatc gcgactgcct ctctttagaa tacacgttcg gtgtatggta gccatataca
     3841 gaaatggagg tgaatcaaat gatgattaat aaagcttata agttttatat ctttccaaat
     3901 caagcacaag caattctaat caataaaacg attggttgtt ctcgctttgt attcaatcat
     3961 tttctttcct tatgggatga cttatataaa gaggcaggaa gaggtttgac ctatggtaca
     4021 tgttctgcca aagttcctgc catgaagaaa gagtttgttt ggctaaaaga agtggatatt
     4081 attgtgattc atttgtctgt ttataatctt gcagatgcgt atacacgctt tttcaaaaaa
     4141 taaaacagcg cctcgcgctt taaatctaag aagaacaacg tacaatcata taccacaamc
     4201 gaaaacattg ccagtgtagg taaaaaataa agttgccgaa agtcgcgaag taaagggacg
     4261 tattgtaaat gctacagtta gacggaaccc ttctggcata tactttgttt cattgttagt
     4321 tgaaacagaa gtacaagaac ttccgaaaac acattcttac atcagaatgg atatgggact
     4381 aaaagatttt gccattttgt cagatggaac aacctataaa aatccgtaat tttttcgctc
     4441 attagaagcg aaggtggcga aagtacagca tgttctttct aga
//
