LOCUS       V00618                  1300 bp ds-DNA     linear   BCT 18-APR-2005
DEFINITION  Transposon Tn5 fragment encoding neomycin and kanamycin resistance
            (neo) and a fragment of the reading frame of a further protein.
ACCESSION   V00618
VERSION     V00618.1  gi:43748
KEYWORDS    drug resistance gene; neomycin resistance; transferase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Beck, E., Ludwig, G., Auerswald, E.A., Reiss, B., Schaller, H.
  TITLE     Nucleotide sequence and exact localization of the neomycin
            phosphotransferase gene from transposon Tn5
  JOURNAL   Gene 19 (3), 327-336 (1982)
  PUBMED    6295884
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     CDS             151..945
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="CAA23892.1"
                     /db_xref="GI:43749"
                     /db_xref="GOA:P00552"
                     /db_xref="InterPro:IPR002575"
                     /db_xref="InterPro:IPR011009"
                     /db_xref="PDB:1ND4"
                     /db_xref="UniProtKB/Swiss-Prot:P00552"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             942..>1300
                     /note="unnamed protein product; unidentified reading
                     frame"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23893.1"
                     /db_xref="GI:581280"
                     /db_xref="GOA:Q99399"
                     /db_xref="HSSP:1EWJ"
                     /db_xref="InterPro:IPR000335"
                     /db_xref="UniProtKB/TrEMBL:Q99399"
                     /translation="MSGTLGFEMTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWM
                     ILQRGDLMLEFFAHPGLDPLASWFSCCLRLDDLAEFYRQCKSVGIQETSSGYPRIHAP
                     ELQEWGGTMAALVDPDGT"
ORIGIN      
        1 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
       61 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
      121 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      181 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      241 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      301 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      361 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      421 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      481 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      541 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      601 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      661 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
      721 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
      781 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
      841 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
      901 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
      961 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     1021 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1081 agcgcgggga tctcatgctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt
     1141 ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg
     1201 tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt
     1261 ggggaggcac gatggccgct ttggtcgacc cggacgggac
//
