LOCUS       ECOMANAA                1604 bp ds-DNA     linear   BCT 15-SEP-1989
DEFINITION  E.coli manA gene encoding phosphomannose isomerase, complete cds.
ACCESSION   M15380
VERSION     M15380.1  gi:146721
KEYWORDS    isomerase; mannose; phosphomannose isomerase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Miles, J.S., Guest, J.R.
  TITLE     Nucleotide sequence and transcriptional start point of the
            phosphomannose isomerase gene (manA) of Escherichia coli
  JOURNAL   Gene 32 (1-2), 41-48 (1984)
  PUBMED    6397402
COMMENT     Original source text: E.coli (strain GM242) DNA, clone pGS57.
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     mRNA            1..1604
                     /product="PMI mRNA"
                     /transcription="CCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTTCAAATTCAGATACGCTAACGTGTTCGCTGGTTAGCAGGTAATACTCAGTATCATCTTTTTTGAGTGGAAAAGGAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAATCCGTCCAGCCAGCCGATGGCCGAGCTGTGGATGGGCGCACATCCGAAAAGCAGTTCACGAGTGCAGAATGCCGCCGGAGATATCGTTTCACTGCGTGATGTGATTGAGAGTGATAAATCGACTCTGCTCGGAGAGGCCGTTGCCAAACGCTTTGGCGAACTGCCTTTCCTGTTCAAAGTATTATGCGCAGCACAGCCACTCTCCATTCAGGTTCATCCAAACAAACACAATTCTGAAATCGGTTTTGCCAAAGAAAATGCCGCAGGTATCCCGATGGATGCCGCCGAGCGTAACTATAAAGATCCTAACCACAAGCCGGAGCTGGTTTTTGCGCTGACGCCTTTCCTTGCGATGAACGCGTTTCGTGAATTTTCCGAGATTGTCTCCCTACTCCAGCCGGTCGCAGGTGCACATCCGGCGATTGCTCACTTTTTACAACAGCCTGATGCCGAACGTTTAAGCGAACTGTTCGCCAGCCTGTTGAATATGCAGGGTGAAGAAAAATCCCGCGCGCTGGCGATTTTAAAATCGGCCCTCGATAGCCAGCAGGGTGAACCGTGGCAAACGATTCGTTTAATTTCTGAATTTTACCCGGAAGACAGCGGTCTGTTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTT"
     gene            392..1567
                     /gene="manA"
     CDS             392..1567
                     /gene="manA"
                     /note="phosphomannose isomerase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24109.1"
                     /db_xref="GI:146722"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
ORIGIN      
        1 ccggacgcag catgaacgac gcatcatgaa acgcctggcg cgccaacaga gttaacgctt
       61 cgggtgcgac tttcaaaatc tcctgccctt caaattcaga tacgctaacg tgttcgctgg
      121 ttagcaggta atactcagta tcatcttttt tgagtggaaa aggagcctga taatgaaagg
      181 gtttgtttga cattgttctc tcacttactg cctggtttgg ttatgctctg ggcgggtgtt
      241 ccgttgccct gttaaaagcg agtaacaata tcctacacac ttttttaaca aaaactgaga
      301 ctagtacgac tttttgcggc tccaggttac ttcccgtagg attcttgctt taatagtggg
      361 attaatttcc acattaaaac agggattgat catgcaaaaa ctcattaact cagtgcaaaa
      421 ctatgcctgg ggcagcaaaa cggcgttgac tgaactttat ggtatggaaa atccgtccag
      481 ccagccgatg gccgagctgt ggatgggcgc acatccgaaa agcagttcac gagtgcagaa
      541 tgccgccgga gatatcgttt cactgcgtga tgtgattgag agtgataaat cgactctgct
      601 cggagaggcc gttgccaaac gctttggcga actgcctttc ctgttcaaag tattatgcgc
      661 agcacagcca ctctccattc aggttcatcc aaacaaacac aattctgaaa tcggttttgc
      721 caaagaaaat gccgcaggta tcccgatgga tgccgccgag cgtaactata aagatcctaa
      781 ccacaagccg gagctggttt ttgcgctgac gcctttcctt gcgatgaacg cgtttcgtga
      841 attttccgag attgtctccc tactccagcc ggtcgcaggt gcacatccgg cgattgctca
      901 ctttttacaa cagcctgatg ccgaacgttt aagcgaactg ttcgccagcc tgttgaatat
      961 gcagggtgaa gaaaaatccc gcgcgctggc gattttaaaa tcggccctcg atagccagca
     1021 gggtgaaccg tggcaaacga ttcgtttaat ttctgaattt tacccggaag acagcggtct
     1081 gttctccccg ctattgctga atgtggtgaa attgaaccct ggcgaagcga tgttcctgtt
     1141 cgctgaaaca ccgcacgctt acctgcaagg cgtggcgctg gaagtgatgg caaactccga
     1201 taacgtgctg cgtgcgggtc tgacgcctaa atacattgat attccggaac tggttgccaa
     1261 tgtgaaattc gaagccaaac cggctaacca gttgttgacc cagccggtga aacaaggtgc
     1321 agaactggac ttcccgattc cagtggatga ttttgccttc tcgctgcatg accttagtga
     1381 taaagaaacc accattagcc agcagagtgc cgccattttg ttctgcgtcg aaggcgatgc
     1441 aacgttgtgg aaaggttctc agcagttaca gcttaaaccg ggtgaatcag cgtttattgc
     1501 cgccaacgaa tcaccggtga ctgtcaaagg ccacggccgt ttagcgcgtg tttacaacaa
     1561 gctgtaagag cttactgaaa aaattaacat ctcttgctaa gctt
//
