LOCUS       KPNBXN                  1212 bp ds-DNA     linear   BCT 01-SEP-1988
DEFINITION  K.pneumoniae bromoxynil-specific nitrilase (bxn) gene, complete
            cds.
ACCESSION   J03196
VERSION     J03196.1  gi:149174
KEYWORDS    bromoxynil-specific nitrilase.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Klebsiella.
REFERENCE   1
  AUTHORS   Stalker, D.M., Malyj, L.D., McBride, K.E.
  TITLE     Purification and properties of a nitrilase specific for the
            herbicide bromoxynil and corresponding nucleotide sequence analysis
            of the bxn gene
  JOURNAL   J. Biol. Chem. 263 (13), 6310-6314 (1988)
  PUBMED    2834373
COMMENT     Original source text: K.pneumoniae DNA, clone pBrx9.; Draft entry
            and printed copy of sequence for [1] kindly provided by
            D.M.Stalker, 02/12/88.
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:573"
     CDS             66..1115
                     /note="bromoxynil-specific nitrilase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA25057.1"
                     /db_xref="GI:149175"
                     /translation="MDTTFKAAAVQAEPVWMDAAATADKTVTLVAKAAAAGAQLVAFP
                     ELWIPGYPGFMLTHNQTETLPFIIKYRKQAIAADGPEIEKIRCAAQEHNIALSFGYSE
                     RAGRTLYMSQMLIDADGITKIRRRKLKPTRFERELFGEGDGSDLQVAQTSVGRVGALN
                     CAENLQSLNKFALAAEGEQIHISAWPFTLGSPVLVGDSIGAINQVYAAETGTFVLMST
                     QVVGPTGIAAFEIEDRYNPNQYLGGGYARIYGPDMQLKSKSLSPTEEGIVYAEIDLSM
                     LEAAKYSLDPTGHYSRPDVFSVSINRQRQPAVSEVIDSNGDEDPRAACEPDEGDREVV
                     ISTAIGVLPRYCGHS"
ORIGIN      
        1 ctgcaggata gtaggggctt gaagagatac gctgtttgtc gagccatcaa aataagggga
       61 ttttcatgga caccactttc aaagcagccg ctgttcaggc cgaaccggta tggatggatg
      121 ccgctgcaac agccgataag accgtgacgc tagtagctaa agccgcagcg gctggcgcgc
      181 agctcgtcgc atttcccgaa ttgtggattc cgggctaccc aggattcatg ctcacgcaca
      241 accaaaccga aaccctacca ttcatcatta aataccgcaa gcaggcaatc gccgccgatg
      301 gaccagaaat cgaaaaaatt cgctgcgcgg ctcaggagca taacattgcg ctctcctttg
      361 ggtacagcga acgggctggc cgtacgctct acatgtcaca aatgcttatc gatgccgatg
      421 gcatcaccaa aattcgtcgt cgaaagctca aaccaacccg ctttgaacga gaactctttg
      481 gcgaaggtga cggatcggac ttacaggtcg cccaaactag cgttggtcgg gtgggtgccc
      541 tcaactgcgc ggagaatttg cagtcgctaa acaagtttgc gcttgctgcc gagggtgaac
      601 agatacatat ctccgcctgg ccattcacgc ttggaagccc tgtgctcgtc ggagactcca
      661 tcggcgccat caaccaggtc tacgcggccg agacggggac cttcgttctc atgtcgacgc
      721 aggtggttgg accgaccggc atcgccgcct tcgagatcga agacaggtac aacccgaatc
      781 agtatcttgg tggtgggtac gcgcggatct acgggcctga catgcagttg aagagcaagt
      841 cgttgtcacc gaccgaagag ggcatcgtct acgccgagat cgacctgtcg atgcttgagg
      901 cagcaaagta ctcgctcgat cccacgggcc actattcgcg ccctgatgtg ttcagcgtgt
      961 cgattaaccg gcaacggcag cctgcggtgt cagaagttat cgactcaaac ggtgacgagg
     1021 acccgagagc agcatgcgag cccgacgagg gggatcgtga ggtcgtaatc tctacggcaa
     1081 taggggttct accccgttat tgcggacatt cctaataaaa agagacacgt tgtaccaaag
     1141 gggtgttcat gtccagacgc agaaaatata gcccagagtt aaaacgcgaa gccatcgctt
     1201 taacccgtca ac
//
