LOCUS       X15545                   474 bp ds-DNA     linear   BCT 23-JUN-1996
DEFINITION  Bacillus amyloliquefaciens barstar gene.
ACCESSION   X15545
VERSION     X15545.1  gi:1155006
KEYWORDS    barstar; ribonuclease inhibitor.
SOURCE      Bacillus amyloliquefaciens (Bacillus velezensis)
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Hartley, R.W.
  TITLE     Barnase and barstar. Expression of its cloned inhibitor permits
            expression of a cloned ribonuclease
  JOURNAL   J. Mol. Biol. 202 (4), 913-915 (1988)
  PUBMED    3050134
REFERENCE   2
  AUTHORS   Hartley, R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-1996) R.Hartley, LCDB/NIDDK, NIH, Bethesda, 20892
            USA, email:hartleyr@helix.nih.gov
  REMARK    Revised by author
COMMENT     On Jan 15, 1996 this sequence version replaced gi:39311.; See also
            acc# x12871.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Bacillus amyloliquefaciens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1390"
                     /clone="pMT311"
     regulatory      94..99
                     /regulatory_class="promoter"
                     /note="-10 region"
     regulatory      109..119
                     /regulatory_class="ribosome_binding_site"
                     /note="pot. ribosome binding site"
     CDS             124..396
                     /note="unnamed protein product; barstar (AA 1 - 90)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA33551.1"
                     /db_xref="GI:39312"
                     /db_xref="GOA:P11540"
                     /db_xref="InterPro:IPR000468"
                     /db_xref="PDB:1A19"
                     /db_xref="PDB:1AB7"
                     /db_xref="PDB:1AY7"
                     /db_xref="PDB:1B27"
                     /db_xref="PDB:1B2S"
                     /db_xref="PDB:1B2U"
                     /db_xref="PDB:1B3S"
                     /db_xref="PDB:1BGS"
                     /db_xref="PDB:1BRS"
                     /db_xref="PDB:1BTA"
                     /db_xref="PDB:1BTB"
                     /db_xref="PDB:1L1K"
                     /db_xref="PDB:1X1U"
                     /db_xref="PDB:1X1W"
                     /db_xref="PDB:1X1X"
                     /db_xref="PDB:1X1Y"
                     /db_xref="PDB:2HXX"
                     /db_xref="PDB:2ZA4"
                     /db_xref="PDB:3DA7"
                     /db_xref="UniProtKB/Swiss-Prot:P11540"
                     /translation="MKKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTG
                     WVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS"
ORIGIN      
        1 gtccaatctg cagccgtccg agacaggagg acatcgtcca gctgaaaccg gggcagaatc
       61 cggccatttc tgaagagaaa aatggtaaac tgatagaata aaatcataag aaaggagccg
      121 cacatgaaaa aagcagtcat taacggggaa caaatcagaa gtatcagcga cctccaccag
      181 acattgaaaa aggagcttgc ccttccggaa tactacggtg aaaacctgga cgctttatgg
      241 gattgtctga ccggatgggt ggagtacccg ctcgttttgg aatggaggca gtttgaacaa
      301 agcaagcagc tgactgaaaa tggcgccgag agtgtgcttc aggttttccg tgaagcgaaa
      361 gcggaaggct gcgacatcac catcatactt tcttaatacg atcaatggga gatgaacaat
      421 atggaaacac aaaccctcag ctatccgatt ggagaatatc agccgcctga atcg
//
