LOCUS       X12871                   429 bp ds-DNA     linear   BCT 14-NOV-2006
DEFINITION  Bacillus amyloliquefaciens gene for barnase (extracellular
            ribonuclease).
ACCESSION   X12871
VERSION     X12871.1  gi:39309
KEYWORDS    barnase; ribonuclease.
SOURCE      Bacillus amyloliquefaciens (Bacillus velezensis)
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1
  AUTHORS   Hartley, R.W.
  TITLE     Barnase and barstar. Expression of its cloned inhibitor permits
            expression of a cloned ribonuclease
  JOURNAL   J. Mol. Biol. 202 (4), 913-915 (1988)
  PUBMED    3050134
COMMENT     See also acc# x15545.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Bacillus amyloliquefaciens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1390"
                     /clone="pMT311"
     CDS             <1..333
                     /codon_start=1
                     /transl_table=11
                     /product="barnase"
                     /protein_id="CAA31365.1"
                     /db_xref="GI:39310"
                     /db_xref="GOA:P00648"
                     /db_xref="InterPro:IPR000026"
                     /db_xref="InterPro:IPR001887"
                     /db_xref="InterPro:IPR016191"
                     /db_xref="PDB:1A2P"
                     /db_xref="PDB:1B20"
                     /db_xref="PDB:1B21"
                     /db_xref="PDB:1B27"
                     /db_xref="PDB:1B2S"
                     /db_xref="PDB:1B2U"
                     /db_xref="PDB:1B2X"
                     /db_xref="PDB:1B2Z"
                     /db_xref="PDB:1B3S"
                     /db_xref="PDB:1BAN"
                     /db_xref="PDB:1BAO"
                     /db_xref="PDB:1BGS"
                     /db_xref="PDB:1BNE"
                     /db_xref="PDB:1BNF"
                     /db_xref="PDB:1BNG"
                     /db_xref="PDB:1BNI"
                     /db_xref="PDB:1BNJ"
                     /db_xref="PDB:1BNR"
                     /db_xref="PDB:1BNS"
                     /db_xref="PDB:1BRG"
                     /db_xref="PDB:1BRH"
                     /db_xref="PDB:1BRI"
                     /db_xref="PDB:1BRJ"
                     /db_xref="PDB:1BRK"
                     /db_xref="PDB:1BRN"
                     /db_xref="PDB:1BRS"
                     /db_xref="PDB:1BSA"
                     /db_xref="PDB:1BSB"
                     /db_xref="PDB:1BSC"
                     /db_xref="PDB:1BSD"
                     /db_xref="PDB:1BSE"
                     /db_xref="PDB:1FW7"
                     /db_xref="PDB:1RNB"
                     /db_xref="PDB:1X1U"
                     /db_xref="PDB:1X1W"
                     /db_xref="PDB:1X1X"
                     /db_xref="PDB:1X1Y"
                     /db_xref="PDB:1YVS"
                     /db_xref="PDB:2C4B"
                     /db_xref="PDB:2F4Y"
                     /db_xref="PDB:2F56"
                     /db_xref="PDB:2F5M"
                     /db_xref="PDB:2F5W"
                     /db_xref="PDB:2KF3"
                     /db_xref="PDB:2KF4"
                     /db_xref="PDB:2KF5"
                     /db_xref="PDB:2KF6"
                     /db_xref="PDB:2ZA4"
                     /db_xref="PDB:3DA7"
                     /db_xref="PDB:3KCH"
                     /db_xref="UniProtKB/Swiss-Prot:P00648"
                     /translation="AQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLAD
                     VAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDH
                     YQTFTKIR"
     mat_peptide     <1..330
                     /product="mature barnase (AA 1 - 110)"
                     /peptide="AQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR"
ORIGIN      
        1 gcacaggtta tcaacacgtt tgacggggtt gcggattatc ttcagacata tcataagcta
       61 cctgataatt acattacaaa atcagaagca caagccctcg gctgggtggc atcaaaaggg
      121 aaccttgcag acgtcgctcc ggggaaaagc atcggcggag acatcttctc aaacagggaa
      181 ggcaaactcc cgggcaaaag cggacgaaca tggcgtgaag cggatattaa ctatacatca
      241 ggcttcagaa attcagaccg gattctttac tcaagcgact ggctgattta caaaacaacg
      301 gaccattatc agacctttac aaaaatcaga taacgaaaaa aacggcttcc tgcggaggcc
      361 gtttttttca gctttacata aagtgtgtaa taaatttttc ttcaaactct gatcggtcaa
      421 tttcacttt
//
