LOCUS       ECOGUSRABC              7742 bp ds-DNA     linear   BCT 27-JUN-1995
DEFINITION  Escherichia coli K-12 gusRABC operon.
ACCESSION   M14641
VERSION     M14641.1  gi:868017
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Jefferson, R.A., Burgess, S.M., Hirsh, D.
  TITLE     beta-Glucuronidase from Escherichia coli as a gene-fusion marker
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8447-8451 (1986)
  PUBMED    3534890
REFERENCE   2
  AUTHORS   Wilson, K.J., Jefferson, R.A.
  TITLE     The GusR repressor of Esherichia coli
  JOURNAL   Unpublished
REFERENCE   3
  AUTHORS   Liang, W.J., Jefferson, R.A.
  TITLE     The gusB gene of Esherichia coli
  JOURNAL   Unpublished
REFERENCE   4
  AUTHORS   Liang, W.J., Wilson, K.J., Jefferson, R.A.
  TITLE     The gus genes of Esherichia coli
  JOURNAL   Unpublished
REFERENCE   5
  AUTHORS   Jefferson, R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-1994) Richard A. Jefferson, Center for the
            Application of Molecular Biology to International Agriculture, GPO
            Box 3200, Canberra ACT 2601, Australia
COMMENT     On Jun 21, 1995 this sequence version replaced gi:148121.
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     CDS             <1..264
                     /codon_start=1
                     /transl_table=11
                     /product="7alpha-hydroxysteroid dehydrogenase"
                     /protein_id="AAA68921.1"
                     /db_xref="GI:868018"
                     /translation="LVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQH
                     TPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            485..1075
                     /gene="gusR"
     CDS             485..1075
                     /gene="gusR"
                     /note="start codon is uncertain, could be 488 or 497"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide repressor"
                     /protein_id="AAA68922.1"
                     /db_xref="GI:868019"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1466..3274
                     /gene="gusA"
     CDS             1466..3274
                     /gene="gusA"
                     /note="also known as uidA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronidase"
                     /protein_id="AAA68923.1"
                     /db_xref="GI:868020"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA
                     HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3271..4644
                     /gene="gusB"
     CDS             3271..4644
                     /gene="gusB"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide permease"
                     /protein_id="AAA68924.1"
                     /db_xref="GI:868021"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4695..5945
                     /gene="gusC"
     CDS             4695..5945
                     /gene="gusC"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protein"
                     /protein_id="AAA68925.1"
                     /db_xref="GI:868022"
                     /translation="MAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRADKPS
                     AGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFA
                     LPGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALT
                     PNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSL
                     GASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQ
                     LTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTP
                     QFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGREKRD
                     GSTLGDRLNYWDVKMSIQYDFMLK"
     CDS             complement(6324..7673)
                     /note="ORF downstream of manA"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA68926.1"
                     /db_xref="GI:868023"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDAKPSPFPGRRKVVRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALSRQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVARKVIRKIKRKNWRNSKLKVHQQWGRCSV"
ORIGIN      
        1 ctggtcagaa atatggcgtt tgacctgggt gaaaaaaata ttcgggtaaa tggcattgcg
       61 ccgggggcaa tattaaccga tgccctgaaa tccgttatta caccagaaat tgaacaaaaa
      121 atgttacagc acacgccgat cagacgtctg ggccaaccgc aagatattgc taacgcagcg
      181 ctgttccttt gctcgcctgc tgcgagctgg gtaagcggac aaattctcac cgtctccggt
      241 ggtggggtac aggagctcaa ttaatacact aacggaccgg taaacaaccg tgcgtgttgt
      301 ttaccgggat aaactcatca acgtctctgc taaataactg gcagccaaat cacggctatt
      361 ggttaaccaa tttcagagtg aaaagtatac gaatagagtg tgccttcgca ctattcaaca
      421 gcaatgatag gcgctcacct gacaacgcgg taaactagtt attcacgcta actataatgg
      481 tttaatgatg gataacatgc agactgaagc acaaccgaca cggacccgga tcctcaatgc
      541 tgccagagag attttttcag aaaatggatt tcacagtgcc tcgatgaaag ccatctgtaa
      601 atcttgcgcc attagtcccg ggacgctcta tcaccatttc atctccaaag aagccttgat
      661 tcaggcgatt atcttacagg accaggagag ggcgctggcc cgtttccggg aaccgattga
      721 agggattcat ttcgttgact atatggtcga gtccattgtc tctctcaccc atgaagcctt
      781 tggacaacgg gcgctggtgg ttgaaattat ggcggaaggg atgcgtaacc cacaggtcgc
      841 cgccatgctt aaaaataagc atatgacgat cacggaattt gttgcccagc ggatgcgtga
      901 tgcccagcaa aaaggcgaga taagcccaga catcaacacg gcaatgactt cacgtttact
      961 gctggatctg acctacggtg tactggccga tatcgaagcg gaagacctgg cgcgtgaagc
     1021 gtcgtttgct cagggattac gcgcgatgat tggcggtatc ttaaccgcat cctgattctc
     1081 tctctttttc ggcgggctgg tgataactgt gcccgcgttt catatcgtaa tttctctgtg
     1141 caaaaattat ccttcccggc ttcggagaat tccccccaaa atattcactg tagccatatg
     1201 tcatgagagt ttatcgttcc caatacgctc gaacgaacgt tcggttgctt attttatggc
     1261 ttctgtcaac gctgttttaa agattaatgc gatctatatc acgctgtggg tattgcagtt
     1321 tttggttttt tgatcgcggt gtcagttctt tttatttcca tttctcttcc atgggtttct
     1381 cacagataac tgtgtgcaac acagaattgg ttaactaatc agattaaagg ttgaccagta
     1441 ttattatctt aatgaggagt cccttatgtt acgtcctgta gaaaccccaa cccgtgaaat
     1501 caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
     1561 gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
     1621 cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt atcagcgcga
     1681 agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac
     1741 tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
     1801 gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
     1861 ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
     1921 aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg ggatccatcg
     1981 cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
     2041 tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt
     2101 cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg
     2161 gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact
     2221 gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg
     2281 gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct actttactgg
     2341 ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg tgctgatggt
     2401 gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc
     2461 ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac
     2521 tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa
     2581 agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat
     2641 taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa
     2701 cgaaccggat acccgtccgc aagtgcacgg gaatatttcg ccactggcgg aagcaacgcg
     2761 taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg acgctcacac
     2821 cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg gatggtatgt
     2881 ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc tggcctggca
     2941 ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt tagccgggct
     3001 gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc tggatatgta
     3061 tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga atttcgccga
     3121 ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga tcttcactcg
     3181 cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg gcatgaactt
     3241 cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac aactctcctg gcgcaccatc
     3301 gtcggctaca gcctcggtga cgtcgccaat aacttcgcct tcgcaatggg ggcgctcttc
     3361 ctgttgagtt actacaccga cgtcgctggc gtcggtgccg ctgcggcggg caccatgctg
     3421 ttactggtgc gggtattcga tgccttcgcc gacgtctttg ccggacgagt ggtggacagt
     3481 gtgaataccc gctggggaaa attccgcccg tttttactct tcggtactgc gccgttaatg
     3541 atcttcagcg tgctggtatt ctgggtgccg accgactgga gccatggtag caaagtggtg
     3601 tatgcatatt tgacctacat gggcctcggg ctttgctaca gcctggtgaa tattccttat
     3661 ggttcacttg ctaccgcgat gacccaacaa ccacaatccc gcgcccgtct gggcgcggct
     3721 cgtgggattg ccgcttcatt gacctttgtc tgcctggcat ttctgatagg accgagcatt
     3781 aagaactcca gcccggaaga gatggtgtcg gtataccatt tctggacaat tgtgctggcg
     3841 attgccggaa tggtgcttta cttcatctgc ttcaaatcga cgcgtgagaa tgtggtacgt
     3901 atcgttgcgc agccgtcatt gaatatcagt ctgcaaaccc tgaaacggaa tcgcccgctg
     3961 tttatgttgt gcatcggtgc gctgtgtgtg ctgatttcga cctttgcggt cagcgcctcg
     4021 tcgttgttct acgtgcgcta tgtgttaaat gataccgggc tgttcactgt gctggtactg
     4081 gtgcaaaacc tggttggtac tgtggcatcg gcaccgctgg tgccggggat ggtcgcgagg
     4141 atcggtaaaa agaatacctt cctgattggc gctttgctgg gaacctgcgg ttatctgctg
     4201 ttcttctggg tttccgtctg gtcactgccg gtggcgttgg ttgcgttggc catcgcttca
     4261 attggtcagg gcgttaccat gaccgtgatg tgggcgctgg aagctgatac cgtagaatac
     4321 ggtgaatacc tgaccggcgt gcgaattgaa gggctcacct attcactatt ctcatttacc
     4381 cgtaaatgcg gtcaggcaat cggaggttca attcctgcct ttattttggg gttaagcgga
     4441 tatatcgcca atcaggtgca aacgccggaa gttattatgg gcatccgcac atcaattgcc
     4501 ttagtacctt gcggatttat gctactggca ttcgttatta tctggtttta tccgctcacg
     4561 gataaaaaat tcaaagaaat cgtggttgaa attgataatc gtaaaaaagt gcagcagcaa
     4621 ttaatcagcg atatcactaa ttaatattca ataaaaataa tcagaacatc aaaggtgcaa
     4681 ctatgagaaa aatagtggcc atggccgtta tttgcctgac ggctgcctct ggccttacct
     4741 ctgcttatgc ggcgcaactg gctgacgatg aagcgggact acgcatcaga ctgaaaaacg
     4801 aattgcgcag ggcggataag cccagtgctg gcgcgggaag agatatttac gcatgggtac
     4861 agggaggatt gctcgatttc aatagtggtt attattccaa tattattggc gttgaaggcg
     4921 gggcgtatta tgtttataaa ttaggtgctc gtgctgatat gagtacccgg tggtatcttg
     4981 atggtgataa aagttttgct ttgcccgggg cagtaaaaat aaaacccagt gaaaatagcc
     5041 tgcttaaatt aggtcgcttc gggacggatt atagttatgg tagcttacct tatcgtattc
     5101 cgttaatggc tggcagttcg caacgtacat taccgacagt ttctgaagga gcattaggtt
     5161 attgggcttt aacaccaaat attgatctgt ggggaatgtg gcgttcacga gtatttttat
     5221 ggactgattc aacaaccggt attcgtgatg aaggggtgta taacagccag acgggaaaat
     5281 acgataaaca tcgcgcacgt tcttttttag ccgccagttg gcatgatgat accagtcgct
     5341 attctctggg ggcatcggta cagaaagatg tttccaatca gatacaaagt attctcgaga
     5401 aaagcatacc gctcgacccg aattatacgt tgaaagggga gttgctcggc ttttacgcgc
     5461 agctcgaagg tttaagtcgt aataccagcc agcccaatga aacggcgttg gttagtggac
     5521 aattgacctg gaatgcgccg tggggaagtg tatttggcag tggtggttat ttgcgccatg
     5581 caatgaatgg tgccgtggtg gataccgaca ttggctatcc cttttcatta agtcttgatc
     5641 gtaaccgtga aggaatgcag tcctggcaat tgggcgtcaa ctatcgttta acgccgcaat
     5701 ttacgctgac atttgcaccg attgtgactc gcggctatga atccagtaaa cgagatgtgc
     5761 ggattgaagg cacgggtatc ttaggtggta tgaactatcg ggtcagcgaa gggccgttac
     5821 aagggatgaa tttctttctt gctgccgata aagggcggga aaagcgcgat ggcagtacgc
     5881 tgggcgatcg cctgaattac tgggatgtga aaatgagtat tcagtatgac tttatgctga
     5941 agtaaaaaat aacgccggag agaaaaatct ccggcgtttc agattgttga caaagtgccg
     6001 ttttttatgc cggatgcggc taaacgcctt atccagccta caaaaactca taaattcaaa
     6061 gtgttgcagg aaaaggtagg cctgataagc gtagcgcatc aggcaatctc tggtttgttt
     6121 tcagatgaaa acgccggagt gaaaattctc cggcgttttg gccgtgaatt actgctgcgg
     6181 aattgccggt acagccggaa cgttaagagc tggcatcgca aacatgccaa caaaatcttc
     6241 taacgacatt ttctgcccat ttaacgttat ctgaccgtta gcatattgca ggctggtggt
     6301 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
     6361 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcct cgctacctga
     6421 gtcataaact cagttgccat atccaccgga atggtcagtt tcgcatccag agatttaacc
     6481 gaacgatcta cttcctgcgc cagcgtttgc ggcgcttctt tagtcgttgc cggatctttc
     6541 aggaacagcg acagattcag ggcactttca ccctgactgt ttttccagct tagcggcgcg
     6601 atagtaatca ccggatcgcc tttcagcatc agcggcaggg cgctaaagaa ggcttccgtc
     6661 actttctcct gataaagttc ggggttgttg gcaatttctg gctgtcgcga cagcgcctga
     6721 gtttgcgcgt tatattgctg gctaaactga tgccaggctt caccatcaat ctggccgact
     6781 tttaaagtca gcttgccgct gcccagatcc tgattctgta ccttcaggct gtttagcgag
     6841 taatccagtt ggctattgat cgttttaccg tcattgacca gatccgattt accgctgatc
     6901 tccatgcctt ccagcagtgc cagttctttg ccttccactg aaatggtcat tttttccagt
     6961 gacagttttt gatttcctac acgctcacca aaacttgcca gcgtgctgga accgtcggtt
     7021 ttcagattat taaaggtcaa ctgcactttc tggttgtatt cgttaactgc gtctatccga
     7081 accactttgc gcctccccgg aaagggagat ggctttgcgt ctctgtcagc atttaactgg
     7141 aactcgccgc cgctaaaggc gactttttca tccttttgct cgtaattcag tggcttgagc
     7201 gaaatatcgg aactggaatc accgctgtaa ccaatgcgcg agttaatctc aaaaggcgtt
     7261 tcaccttttg ccatatcaaa cagtggtttg cttacttcgt tattaaccag cgtggtttga
     7321 attgatgcca tcgacgggat caggttcagt tttttaagct gggcaagcgg gaagggacca
     7381 tgatcaaccg attcgttgaa gatgacgctc tgaccgcttt taatccacgg attttctttc
     7441 ccggcaatgg gtttcaccaa cagttgcaac tggctgctga atacgccgcg atgatagttt
     7501 tgataactca cttccaggtt ggattcagga gctgtcagtt tgagttgcgc gttcgcctgc
     7561 gcgaccatgt cttcgagatg ggtttcaatc ttcttgcctg tataccatgc gccgcctgtc
     7621 cagactacgc ctagcgcaac aatgacgcct accgctacca gcgatttatt cataatgatt
     7681 atccataaaa tgaaatcagg cggactggcc gcctgaaggt gttataagcc tttaataagc
     7741 tt
//
