LOCUS       V00087                  2521 bp ds-DNA     linear   BCT 18-MAR-1996
DEFINITION  Agrobacterium tumefaciens gene encoding nopaline synthetase.
            (Resident in plasmid pTiT37.).
ACCESSION   V00087
VERSION     V00087.1  gi:39105
KEYWORDS    nopaline synthetase; plasmid; synthetase.
SOURCE      Agrobacterium tumefaciens (Rhizobium radiobacter)
  ORGANISM  Agrobacterium tumefaciens
            Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
            Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
            Agrobacterium tumefaciens complex.
REFERENCE   1
  AUTHORS   Depicker, A., Stachel, S., Dhaese, P., Zambryski, P., Goodman, H.M.
  TITLE     Nopaline synthase: transcript mapping and DNA sequence
  JOURNAL   J. Mol. Appl. Genet. 1 (6), 561-573 (1982)
  PUBMED    7153689
REFERENCE   2
  AUTHORS   Bevan, M., Barnes, W.M., Chilton, M.D.
  TITLE     Structure and transcription of the nopaline synthase gene region of
            T-DNA
  JOURNAL   Nucleic Acids Res. 11 (2), 369-385 (1983)
  PUBMED    6298724
COMMENT     On Aug 9, 2002 this sequence version replaced gi:154779.; Sequence
            taken from reference [2].
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Agrobacterium tumefaciens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:358"
                     /note="Geneious name: source Agrobacterium tumefaciens"
     misc_difference 190..191
                     /note="GT is GAT in [1]; conflict"
                     /citation="[2]"
                     /note="Geneious name: GT is GAT in [1]; conflict
                     difference"
     misc_difference 378..380
                     /note="GAT is GT in [1]; conflict"
                     /citation="[2]"
                     /note="Geneious name: GAT is GT in [1]; conflict
                     difference"
     mRNA            550..2002
                     /product="messenger RNA"
                     /note="Geneious name: messenger RNA"
     CDS             585..1826
                     /note="unnamed protein product; coding sequence"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23427.1"
                     /db_xref="GI:39106"
                     /db_xref="GOA:P00386"
                     /db_xref="InterPro:IPR003421"
                     /db_xref="InterPro:IPR008927"
                     /db_xref="InterPro:IPR011128"
                     /db_xref="InterPro:IPR013328"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="UniProtKB/Swiss-Prot:P00386"
                     /translation="MAITLSATSLPISAADHHPLPLTVGVLGSGHAGTALAAWFASRH
                     VPTALWAPADHPGSISAIKANEGVITTEGMINGPFRVSACDDLAAVIRSSRVLIIVTR
                     ADVHDSFVNELANFNGELATKDIVVVCGHGFSIKYERQLRFKRIFETDNSPITSKLSD
                     QKKCNVNIKEMKASFGLSCFPIHRDDAGVIDLPEDTKNIFAQLFSARIICIPPLQVLF
                     FSNCITHAVPAVMNIGRLRDPANSLTKRAEKWLLELDERTPRAEKGFFFYGEGSNTYV
                     CNVQEQIDHERRKVAAACGLRLNSLLQECNDEYDTDYETLREYCLAPSPHNVHHACPD
                     NMEHRYFSEELCSLEDVAAIAAIANIELPLTHAFINIIHAGKGKINPTGKSSSVIGNF
                     SSSDLIRFGATHVFNKDEMVE"
     misc_difference 1249
                     /note="G is A in [1]; conflict"
                     /citation="[2]"
                     /note="Geneious name: G is A in [1]; conflict difference"
     misc_difference 1661
                     /note="C is A in [1]; conflict"
                     /citation="[2]"
                     /note="Geneious name: C is A in [1]; conflict difference"
     misc_difference 1668
                     /note="C is A in [1]; conflict"
                     /citation="[2]"
                     /note="Geneious name: C is A in [1]; conflict difference"
ORIGIN      
        1 tagccgaccc agacgagcca agggatcttt ttggaatgct gctccgtcgt caggctttcc
       61 gacgtttggg tggttgaaca gaagtcatta tcgtacggaa tgccaagcac tcccgagggg
      121 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
      181 taaatatccg ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
      241 gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcat gagcggagaa
      301 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga
      361 actgacagaa ccgcaacgat tgaaggagcc actcagccgc gggtttctgg agtttaatga
      421 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
      481 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
      541 tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaatggca attaccttat
      601 ccgcaacttc tttacctatt tccgccgcag atcaccatcc gcttcccttg accgtaggtg
      661 tcctcggttc tggtcacgcg gggactgcat tagcggcttg gttcgcctcc cggcatgttc
      721 ccacggcgct gtgggcacca gcagatcatc caggatcgat ctcagcaatc aaggccaatg
      781 aaggagttat caccaccgag ggaatgatta acggtccatt tagggtctca gcctgtgatg
      841 accttgccgc agttattcgc tccagccgtg tactgattat tgtaacccgt gcggacgttc
      901 acgacagctt cgtcaacgaa ctcgccaact tcaacggcga actcgcaaca aaggatattg
      961 tcgtcgtgtg cggccatggc ttctccatca agtacgagag acagctgcga ttcaagcgaa
     1021 tattcgagac ggataattcg cccataacgt ctaagctatc ggatcaaaaa aaatgtaacg
     1081 tcaacatcaa ggaaatgaaa gcgtctttcg gactgtcatg tttcccaatt catcgcgatg
     1141 atgctggcgt gattgatcta cccgaagata ccaagaacat ctttgcccag ctattttccg
     1201 ctagaatcat ctgcatcccg ccgttgcaag tgctattctt ttccaactgt atcactcatg
     1261 cggttccggc agtcatgaac atcggaagac tccgcgaccc agccaattct cttactaaaa
     1321 gagctgagaa gtggcttctt gaactagacg agcgaacccc acgagccgag aagggctttt
     1381 tcttttatgg tgaaggatcc aacacttacg tttgcaacgt ccaagagcaa atagaccacg
     1441 aacgccggaa ggttgccgca gcgtgtggat tgcgtctcaa ttctctcttg caggaatgca
     1501 atgatgaata tgatactgac tatgaaactt tgagggaata ctgcctagca ccgtcacctc
     1561 ataacgtgca tcatgcatgc cctgacaaca tggaacatcg ctatttttct gaagaattat
     1621 gctcgttgga ggatgtcgcg gcaattgcag ctattgccaa catcgaacta cccctcacgc
     1681 atgcattcat caatattatt catgcgggga aaggcaagat taatccaact ggcaaatcat
     1741 ccagcgtgat tggtaacttc agttccagcg acttgattcg ttttggtgct acccacgttt
     1801 tcaataagga cgagatggtg gagtaaagaa ggagtgcgtc gaagcagatc gttcaaacat
     1861 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     1921 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     1981 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2041 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     2101 atcaaacttc ggtactgtgt aatgacgatg agcaatcgag aggctgacta acaaaaggta
     2161 tgcccaaaaa caacctctcc aaactgtttc gaattggaag tttctgctca tgccgacagg
     2221 cataacttag atattcgcgg gctattccca ctaattcgtc ctgctggttt gcgccaagat
     2281 aaatcagtgc atctccttac aagttcctct gtcttgtgaa atgaactgct gactgccccc
     2341 caagaaagcc tcctcatctc ccagttggcg gcggctgata caccatcgaa aacccacgtc
     2401 cgaacacttg atacatgtgc ctgagaaata ggcctacgtc caagagcaag tcctttctgt
     2461 gctcgtcgga aattcctctc ctgtcagacg gtcgtgcgca tgtcttgcgt tgatgaagct
     2521 t
//
